These data suggest that adhesion strength could potentially serve as a stable marker for migration and metastatic potential within a given tumor population and that the fraction of weakly adherent cells present within a tumor could act as a physical marker for metastatic potential.īiophysical markers, such as cell deformability, are an emerging alternative to assess metastatic potential ( 7–12). In human breast tumors, expression of genes associated with the weakly adherent population resulted in worse progression-free and disease-free intervals. Subpopulations did not show differences in expression of proteins involved in the focal adhesion complex but did exhibit intrinsic focal adhesion assembly as well as contractile differences that resulted from differential expression of genes involved in microtubules, cytoskeleton linkages, and motor activity. Weakly adherent cells displayed increased migration in both two-dimensional and three-dimensional migration assays this was maintained for several days in culture. Adhesion strength is heterogeneous even among cancer cells within a given population, and using a parallel plate flow chamber, we separated and sorted these populations into weakly and strongly adherent groups when cultured under stromal conditions, this adhesion phenotype was stable over multiple days, sorting cycles, and common across all epithelial tumor lines investigated. Common to disseminating cells from such tumors, however, is the need to modulate their adhesion as they detach from the tumor and migrate through stroma to intravasate. Despite significant effort, there is no universal biological marker that serves as a metric for metastatic potential of solid tumors. We’ll be discussing the benefits of T-Peel tests over other adhesion test types as well as a live demonstration of pass/fail criteria.Tumors are heterogeneous and composed of cells with different dissemination abilities. This test is a quick, simple, and repeatable method when establishing quality or safety standards in adhesion strength testing. Commonly known as the T-Peel Test, this type of testing can be found in industries like packaging, automotive, leather and upholstery, food, and beverage and many more. This webinar will focus on the most common approach to measuring the adhesion strength of two materials bonded together with an adherent. Cost: Free to Attend - Registration Required.

How to test adhesion gel in portal 2